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Dark field light microscope lab 3

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created 5 years ago by vriddle
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updated 5 years ago by vriddle

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1

What is principle behind dark field microscopy?

Opposite of light microscopy undiffracted light doesn't pass through specimen resulting in bright image against dark background useful in viewing UNSTAINED organisms

2

When would you use dark field microscopy

Wen observing unstained living microorganisms or microorganisms that are hard to stain and spirochetes that are poorly defined in bright field

3

Bright field

Uses light from lamp under stage to focus in plane of specimen

4

Dark field

Condenser forms hollow cone of light objective lense sits in dark hollow cone and light travels around objective lense like backlighting

5

What is function of abbé condenser in dark field microscopy

Directs light away makes dark

6

Function of dark field stop

To turn the compound microscope into a dark field microscope

7

How is compound mic turned into dark field mic

Fitting it with dark field condenser that has a resolving power greater than the objective
Light now passing through the specimen is diffracted and enters objective lense whereas undiffracted light doesn't results in bright image against dark background


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