Carbs excersise 21 triple sugar Iron Agar test

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1

What is TSI test used for

Identify enteric bacteria and differentiate them from other gram neg rods

2

TSIA

Rich medium differentiates glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction

3

What does TSIA include in addition to 3 carbs

Beef extract
Yeast extract
Peptone
Sodium thiosulfate

4

What is peptone used for

Carbon and nitrogen source

5

What is sodium thiosulfate used for

Source of reducible sulfur

6

What is pH indicator in TSIA

Phenol red

7

What is the hydrogen sulfide indicator in TSIA

The iron in ferrous sulfate

8

How is TSIA medium prepd

Agar slant with deep butt provides aerobic and anaerobic growth enviornments

9

How is TSIA slant inoculated

Stab in agar butt followed by fishtail streak of slant

10

How long is incubation

18-24 hrs for carb ferm
48 hrs for hydrogen sulfide

11

Saccharolytic

Those organisms capable of breaking glycosidic bonds in carbs
(Sugar+lysis)

12

TSI yellow slant/yellow butt

Glucose and lactose and or sucrose fermentation with acid accumulation in slant and butt
A/A

13

What happens when TSI inoculated with glucose only fermenter

Acid products lower pH turn everything yellow I. A few hrs but glucose in short supply(.1) exhausted in 12 hrs

14

What happens as glucose used up

Reversion, takes 18-24 hrs Organisms located in slant (aerobic region) start to break down available amino acids producing NH3 and raising pH

15

Where does reversion occur

Only in slant bc of anaerobic conditio s in butt

16

What does a TSI slant with a red slant and yellow butt after 24 hrs incubation indicate

That organism ferments glucose but not lactose

17

What color is slant if organisms ferment glucose and lactose

Yellow throughout but bc lactose and sucrose 10 higher conc than glucose, more acid produced and slant and butt stay yellow after 24 hrs

18

TSI test result red slant/ yellow butt

Glucose fermentation with acid production
Proteins catabolized aerobically (in slant) with alkaline products (reversion)
K/A

19

TSI test result red slant/red butt

No fermentation
Peptone catabolized aerobically and anaerobically with alkaline oproducts
Not from enterobacteriacae
K/K

20

TSI test result red slant/no change in butt

Organism is growing slowly or not at all
Not from enterobacteriacae
Nc/Nc

21

Black precipitate in agar

Sulfur reduction (an acid condition, from fermentation of glucose or lactose and or sucrose, exists in the butt even if the yellow color is obscured by black precipitate
H2S

22

Cracks in or lifting of agar

Gas production
G

23

What could happen if read results too early

Early- could show yellow throughout leading you to think org is lactose or sucrose fermenter when it simply may not have gotten rid of glucose yet

24

Reading after lactose and sucrose depleted

Would show yellow butt red slant so think org is glucose only fermenter

25

What is purpose of thiosulfate in TSI agar

When bacteria use sodium thiosulfate they can reduce it to sulfite using enzyme thiosulfate reductase and release hydrogen sulfide gas

26

What is meant by term saccharolytic bacteria. What reaction would it give in TSI tube

Uses all three sugars, glucose, lactose, sucrose
Slant would be yellow bc acid formation from breaking glycosidic binds


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