Who introduced into microbiology the procedure of air drying, chemical fixation and aniline dyes?
Staining bacteria enhances the _______ between and the surrounding environment and permits observation of greater detail and resolution than wet mounts do.
Name the various tools you can use to transfer bacteria to a microscope slide.
With simple techniques may involve _______________ in which only _______ ________ is used and all the bacteria are similarly stained.
Multiple reagents being used is called______________ and the bacteria react to the reagents the same or different?
A smear is prepared by:
Fixing denatures bacterial enzymes preventing them from digesting cell parts which cause the cells to break is a process called _____________?
The dry smear is passed through a ____________ several times to __________ the bacteria.
The dyes are usually _______ but some are _____ or ________.
If the chromophore is a positive ion like the previous Methylene blue equation (Methylene blue+)the stain is considered a ______________.
True or false. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
-start with a clean slide
-handle slides by the edge
mark bottom of the slide with a circle to mark where bacteria goes
-sterilize your inoculating loop heating to redness
allow the loop to cool without touching it or setting it down about 30 seconds
-from solid media- put a drop of water in the circle
-from liquid media-place 2 or 3 loopfuls of the liquid culture on the slide with the sterile loop
-allow the smears to dry at room temp
What value is a simple stain?
What is the purpose of heating fixing the smear?
How does alcohol chemically fix the bacteria?
•It removes water, it dehydrates the cells. This is important when mounting the cells in non-aqueous mounting medium.
•It denatures proteins. This way the metabolism of the cell is stopped and the cell dies. The metabolism is dependent on enzymes, which are proteins.
•It dissolves and removes lipids. The cell membrane(s) of the bacteria is harmed by the alcohol.
In heat fixing what would happen if too much heat was applied?
Methylene blue can be prepared as a basic stain or an acidic stain. How much would the pH of the stain affect the staining of bacteria?
Of what value is a simple stain?
Can dyes other than methylene blue be used for direct staining?
Bacteria can be seen without staining. Why is Koch's recommendation for fixing and staining important for microbiology?
Why is the gram stain a very useful stain?
Infection quality control staff in a sterilization unit of a hospital used a simple stain to determine whether bacteria was present in sterilized materials. A simple stain of sterile saline used for respiratory therapy revealed the presence of bacteria. Is the saline contaminated?