streak plate method of isolation
colony forming unit (CFU)
What are streak patterns designed to do?
quadrant streak method
zigzag streak pattern
The identification process of an unknown microbe relies on what?
How do you create a pure culture?
When performing streak plate inoculation, what are the two ways you can hold the plate?
What are the 4 steps in the quadrant streak method using a loop?
1. streak the mixed culture back and forth in one quadrant of the agar plate. flame the loop.
2. rotate the plate 90 degrees and touch the agar in an uninoculated region to cool the loop. flame the loop.
3. rotate the plate nearly 90 degrees and streak again using the same wrist motion. cool loop prior to streaking. flame loop.
4. after cooling the loop, streak one last time into the center of the plate. flame the loop and incubate the plate in an inverted position.
What 2 broth cultures will we use in class for practicing streaking methods?