Excercise 7-3 Micro Lab: Antimicrobial Susceptibility Test

Helpfulness: 0
Set Details Share
created 4 years ago by OceansVoice
show moreless
Page to share:
Embed this setcancel
code changes based on your size selection


natural antimicrobial agents produced by microorganisms


antimicrobials or antimicrobics

all substances used to treat bacterial infections


Kirby Bauer test

also called the disk diffusion test, it measures the effectiveness of antimicrobics against pathogenic microorganisms


How does the Kirby Baur test work?

antimicrobic impregnated paper discs are placed that is inoculated to form a bacterial lawn. the plates are incubated and upon being taken out, if the organism is susceptible to the drug, it will form a zone of inhibition


zone of inhibition

clear zone around the disk where growth has been inhibited



drugs that kill the organism



drugs that stop growth but don't kill the microbe


What is the depth that Kirby Bauer agar is poured and why is it so important the depth?

4mm deep in either a 150mm or 100mm petri dish

depth is important of its effect on diffusion. thick agar slows lateral diffusion and thus produces smaller zones than plates held to the 4mm standard


What kind of agar is used in Kirby Bauer method?

Mueller Hinton agar with a pH of 7.2 to 7.4


In Kirby Bauer method, the broth is diluted to match the _____ turbidity in the McFarland standard before inoculating the plate



How long are the discs containing the antimicrobial agent incubated in the Kirby Bauer method?

16 to 18 hours at 2 degrees Celsius


antimicrobial susceptibility testing

used to measure the effectiveness of antibiotics and other chemotherapeutic agents on pathogenic organisms


how does the antibiotic chloramphenicol work?

prevents peptide bond formation during translation


how does the antibiotic ciprofloxacin work?

interferes with DNA replication


how does the antibiotic trimethoprim work?

inhibits purine and pyrimidine synthesis


how does the antibiotic penicillin work?

inhibits cross linking of the cell wall's peptidoglycan


Bacterial resistance mechanisms to antibiotics can be broken down into three main types:

1. altered target such as antibiotic can no longer interact with cellular process

2. an alteration in how the drug is taken into the cell

3. enzymatic destruction of drug

Related pages

3m coding referencemicroscope with labeled partssagittal view of human braindifference between uracil and thyminewhat happens if the medulla oblongata is damagedeasy notecards reproductive systemlymphoblasts predominate in the bloodcharacterization in the great gatsbyhow does pyruvate enter a mitochondrionwhat is autosome in biologysalmonella typhimurium oxidase teststreaking technique in microbiologyflashcards 1-20sickle cell anemia autosomal recessive or dominantrecombinant dna quizautonomic ganglia containcraniosacral division is another name for theexcretory system physiologyhalstead clampmajor monosaccharide found in the bodyfour postulates of natural selectionidentify the stages of mitosiswhere is the myelin sheath locatedtubular secretion in nephronthe frontal parietal occipital and temporal lobes form theblood clot circulating in the bloodstreamtributyrin agarh2s test microbiologycorynebacterium diphtheriae virulence factorsabdominal girdle musclesbingo en espanoldefinition of visceral pleurareview sheet exercise 22 human cardiovascular physiology answersdna synapsiswhat causes av valves to closedifference between compact and spongy boneat a specific area of a chromosome the sequencethe air compressor governor controlswhat are bacteria using for nutrients in nutrient agarrespiratory passagewaysmoves eyeball superiorlylining of the small intestinewhat are the characteristics of capillariessensible perspiration is produced by what glandsfrom the atrium through the tricuspid valve to thechromosomal abnormalities occur when a zygote's cells haveterminologies in biologychemical equation for synthesiswhat produces the striations of a skeletal muscle celllayers of mucosa2nd smallest planetsize of smallpox virusdescribe how energy flows through ecosystemsontogenetic explanationglucose fermentation test procedurecounting in french 1-30atomic mass of molybdenumwhich statement best describes arteriesreal estate exam flashcardsanatomy and physiology final exam answersurine passes through the ________function of renal tubule in kidneyecg findingsfactors affecting peripheral resistancewhy did you shake the mr vp culturecellular respiration in the presence of oxygenatypical connective tissuewhich of the following is a role of lymph nodescontinental congress apushthe normal pacemaker of the heart is the av nodevenous sinusoids