Exercise 5-4 Micro Lab: Methyl Red and Voges-Proskauer Tests

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Methyl Red and Voges-Proskauer Broth

solution containing only peptone, glucose, and a phosphate buffer

Peptone and glucose provide protein and carbohydrate and the potassium phosphate resists pH changes in the medium


MR test

designed to detect organisms capable of performing mixed acid fermentation which overcomes the phosphate buffer in medium and lowers the pH


Mixed acid fermentation is verified by

the addition of methyl red indicator dye following incubation


Methyl red is ____ at a pH of 4.4 and ______ at a pH of .2. Between these two pH values, it is shades of _____



_______ is the only true indication of a positive result. ____ is negative or inconclusive and __ is negative

red color


the Voges-Proskauer test was designed for

for organisms that were able to ferment glucose but quickly convert their acid products to acetoin and 2,3 butanedoil


adding VP reagents to the medium does what?

oxidizes the acetoin to diacetyl,which in turn reacts with guanidine nuclei from peptone to produce red color

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