Capsule, Endospore, and Flagella Staining

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1

A Smear for the capsule requires heat-fixing.

False

2

The basic stain (Maneval's) is applied and remains for five minutes.

False

3

What is the purpose for the Congo red stain?

It stains the background.

4

What is the purpose of the Maneval's stain?

It colorizes the cell proper

5

Nigrosin can be used in place of Congo red.

True

6

This stain is both a structural and differential stain.

True

7

Identify 3 species of bacteria that may have capsules.

Bacillus anthracis, streptococcus pneumoniae, Klebsiella pneumoniae

8

The rinsing agent in the Schaeffer-Fultron Method of endospore staining is

DI water

9

The stain is both a structural and differential stain

True

10

The primary stain in the endospore stain is:

malachite green

11

What color are the vegetative cells in the endospore stain?

red

12

Heat is not necessary using the Schaeffer-Fulton method.

False

13

A hood is necessary using the Schaeffer-Fulton method

True

14

Identify 2 genera that have endospores.

Bacillus and Clostridium

15

Match the location of the endospore with the descriptive term.

Subterminal endospore- The endospore is between the middle and end of the cell.

Terminal endospore- The endospore is on the end of the cell.

Central endospore- The endospore is in the middle of the cell.

16

This stain is both a structural and differential stain

False

17

Match the flagella arrangement to the definition:

Amphitrichous- Flagella at both ends of the bacterium.

Peritrichous- Flagella covering the entire cell surface of the bacterium.

Monotrichous/polar- A single flagellum found on one end of the bacterium.

Lophotrichous- Tufts of flagella at one end of the bacterium.

18

Why can't flagella be observed in a living sample?

Bacterial flagella are too thin to be observed with the light microscope and ordinary stains. They need to be overstained and this kills them unfortunately.

19

Organisms with a capsule and endospores are easy to destroy.

False

20

All staining methods used in all the staining exercises performed in lab always color or stain the microbe.

False


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