Exercise 5-2 Micro Lab: Oxidation-Fermentation Test

Helpfulness: 0
Set Details Share
created 4 years ago by OceansVoice
372 views
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

The oxidation fermentation test is designed to:

differentiate bacteria on the basis of fermentative or oxidative metabolism of carbohydrates

2

What does Hugh and Leifson's OF test include to reduce the possibility that alkaline products from peptone ulitization will neutralize weak acids produced by oxidation of the carbohydrate?

a high sugar to peptone ratio

3

What is the pH indicator in the OF test?

bromthymol blue dye

4

the bromthymol blue dye is ____ at pH of 6 and ____ at pH of 7

yellow
green

5

How is the OF medium prepared?

with glucose, lactose, sucrose, maltose, mannitol, or xylose and is not slanted

6

After innoculation, what happens to one of the tubes of OF and why?

it is sealed with a layer of mineral oil to promote anaerobic growth and fermentation

7

Organisms that are able to ferment the carbohydrate or ferment and oxidize the carbohydrate will do what?

turn the sealed and unsealed media yellow throughout

8

Organisms that are able to oxidize only will do what?

turn the unsealed medium yellow and leave the sealed medium green or blue

9

Slow or weak fermenters will do what?

turn both tubes slightly yellow at the top

10

Organisms that are not able to metabolize sugar will do what?

produce no color change or turn the medium blue because of alkaline products from amino acid degredation


Related pages


contents of femoral trianglesomatosensory motor cortexhesi test questionsapush chapter 15madam cj walker biography bookwhat is the fascicle arrangement of the orbicularis oris musclehormones travel through the bloodstream to affect othercraniosacral division is another name for thedove soap competitorssarcomere m linedefine segregation biologyhair stylist symbolssecondary succession is most likely to occurwhy is heat necessary in spore stainingnadh moleculestructure of scapula2 phosphoglyceratea large phagocyte found in lymph nodesyellow bone marrow contains a large percentage ofpancreatic enzymes are secreted in response to the hormoneclassification of organisms in the three domains is based onto kill a mockingbird tesglucose broth testwhy is it called the fluid mosaic modelgeometric mean percentagesfetal skull fontanellesanatomy and physiology 1 quizzessavanna temperature rangediarthroticpurpose of the ureterciliates definition biologywhich organelle or structure is absent in plant cellssebaceous glands secretewhy is pyruvate converted to lactate in anaerobic conditionsmass of an electron in amupurpose of cellular respirationwhat is a sensory receptorcardiac contractile cellscontraindications for iontophoresischapter 11 microbiology testwhich vessels supply blood to the myocardiumsignificance of red blood cells being anucleatewhich of the following statements is true regarding absorption costingwhere are osteocytes foundsteps in citric acid cycleosmotolerant organismdamaged thalamusanaphase in mitosisconsecutive interior angle definitionwhat muscle causes goosebumpspictures of flags of countriesamerican dad akikovestibule of the inner earhypothalamus and hormonesamino acids code for proteinprecipitation in the savannathe single most important erosional agent isa prime mover agonistwebster ashburton treaty 1846tortora anatomy bookmonomers and polymers of nucleic acidswhat hormone is involved in diabetes mellitusthe mercurial barometer was invented bymedulla oblongata injurycatabolizedcoxal bone definitionthe modulating control valve allows you to control thewhich of the following statements is true about the glycocalyxplasma cells quizletrenal failure leads to this blood conditionhow to pronounce spanish alphabetcampbell reece ap biology 8th edition