Gene Structure and Replication Chapter 11 set 2

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Chapter 11
Biology 1406, Summer, Dr. Kirk, Test 5
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College: First year
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The mechanism of DNA replication is



Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and allowed to replicate for 1 generation. The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s)?

one half-heavy (intermediate to heavy and light) band


Bacteria are grown in 15N (heavy) medium and then transferred to 14N (light) medium and allowed to replicate for 2 generations. The DNA is subsequently isolated and centrifuged in a CsCl2 gradient to yield what type of gradient band(s)?

one light and one half-heavy band


Which of the following does NOT belong with the others?

DNA ligase


During DNA replication, all of the following proteins are important for separating the DNA strands and allowing movement of the replication fork EXCEPT

DNA polymerase.


How many replication forks are there at a replication origin?



Unlike the leading strand, ______________ are made in replicating the lagging strand of DNA.

Okazaki fragments


Which of the following statements best describes DNA polymerase?

It is an enzyme that requires the presence of a small double-stranded nucleic acid primer to catalyze the addition of nucleotides to the 3 end of a growing DNA strand.


This molecule catalyzes covalent bond formation between fragments of DNA.



All of the following are critical factors for DNA replication on the leading strand EXCEPT?

DNA ligase


Which molecule is responsible for separating double-stranded DNA into single strands?

DNA helicase


____________produces short sequences of RNA, which allows polymerase to synthesize a new strand of DNA.

DNA primase


The function(s) of DNA polymerase includes which of the following?

DNA synthesis, DNA proofreading, and removes RNA primers and fills in the gaps


Which of the following statements about DNA replication is INCORRECT?

It is powered by the hydrolysis of ATP


Which of the following proteins-functions is mismatched?

topoisomerase-separates double-stranded DNA into single strands


Why is there a need to produce Okazaki fragments on the lagging strand, but not on the leading strand of DNA?

The two parental strands of DNA are antiparallel and DNA polymerase makes DNA in the 5' to 3' direction only.


________ attaches DNA sequences at the ends of eukaryotic chromosomes.



Which of the following statements about telomeres is INCORRECT?

Telomeres would predominate more in cells of older than younger individuals.


Where would one expect to find the most telomerase?

male germ cells that give rise to gametes


During the DNA replication process, which of the following organisms would have the fewest origins of replication per cell?

a rapidly dividing Streptococcus bacterium

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