Microbiology Lab Quiz #5, 24

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1

Catalase and oxidase production

enzymes unique to aerobic organisms

2

catalase

enzyme that breaks down an organism's ability to utilize oxygen; degrades hydrogen peroxide to harmless water and oxygen

3

Presence of catalase is visualized by

bubbling of the oxygen gas that is released when catalase is present

4

hallmark of aerobic organisms

ability to degrade poisonous hydrogen peroxide; possess catalase

5

hallmark of anaerobic organisms

inability to degrade poisonous hydrogen peroxide; do not possess catalase

6

oxidase

enzyme that catalyzes transfer of electrons in the cytochrome chain of ETC during aerobic respiration

7

what is oxidase necessary for

for the functioning of the cytochrome c transport reaction of the ETC

8

Presence of oxidase

distinguishes subgroups of chemoautotrophs; allows aerobic organism to degrade certain amines to form colored purple products

9

respiratory enzyme found in most aerobic organisms

catalase

10

chemical reagent used to test for catalase

hydrogen peroxide

11

do not produce catalase and are poisoned by the accumulation of hydrogen peroxide

anaerobic organisms

12

catalase test is valuable in distinguishing between Gram-positive cocci:

Staphylococcus (catalase +)

Streptococcus (catalase -)

13

catalase test is valuable in distinguishing between Gram-positive long rods:

Bacillus (catalase +)

Clostridium (catalase -)

14

Negative test for oxidase

no purple color when streaked onto filter paper; inability of an aerobic organism to oxidize certain amines

15

Catalase test medium

tryptic soy agar

16

Oxidase test medium

filter paper

17

Obligate anaerobe is expected to give this result when tested for oxidase

negative result

18

color that appears in a positive oxidase test

purple

19

chemical reagent added to test for the production of oxidase

Kovac's oxidase reagent

20

positive oxidase test depends upon the presence or absence of

cytochromes in the cell

21

Oxidase production has no value in classification

false

22

catalase and oxidase

enzymes related to an organism's ability to utilize oxygen are characteristics used in classification of bacteria

23

an aerobic organism that can oxidize certain amines to form colored products

has a c type cytochrome


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