Lab manual: Urease Test

Helpfulness: 0
Set Details Share
created 6 years ago by jenkarmata
739 views
book cover
Microbiology
No Chapter
Lab manual Urease test Microbiology 205
updated 6 years ago by jenkarmata
Grade levels:
College: Second year
show moreless
Page to share:
Embed this setcancel
COPY
code changes based on your size selection
Size:
X
Show:
1

Urease is produced by some microorganisms, is an enzyme that is especially helpful in identification of

Proteus vulgaris.

2

Urease is produced by some

microorganisms

3

Although other organisms may produce urease, their action on the substrate urea tends to be slower than that seen w/

Proteus species.

4

Other organisms my produce urease, but their action on ______ ____ tends to be slower than that seen with Proteus species.

substrate urea

5

Is urease an enzyme?

~Yes~

6

The urease test serves rapidly to distinguish members of this genus from other...

non lactose-fermenting enteric microorganisms.

7

Whay type of enzyme is Urease?

Hydrolytic

8

What is the function of Urease (hydrolytic enzyme)

Attack nitrogen & carbon bond in amide compounds such as urea & forms the alkaline end product AMMONIA.

9

What attacks nitrogen & the carbon bond in amide compounds such as urea & forms the alkaline end product ammonia?

Urease (which is a hydrolytic enzyme)

10

Urease attacks _a___ & the _b_______ in __c______ such as urea & forms the alkaline end product __d_____.

a. nitrogen
b. carbon bond
c. amide compounds
d. ammonia

11

The presence of urease is detectable when the organisms are grown in a urea broth medium containing the pH indicator

phenol red

12

As urea is split into it products, the presence of __a___ creates an alkaline environment that causes the the phenol red to turn a deep __b___

a. ammonia
b. pink

13

The presence of ammonia turning the phenol red a deep pink is a ___a_____ reaction for the presence of __b_______.

a. positive
b. urease

14

On a urease test the color yellow means

Negative reaction for the presence of urease.

15

On a urease test the color pink means

Positive reaction for the presence of urease.


Related pages


what happens during dna replicationwhat does cv stand for in statisticsmcdonalds market penetrationwhat is cnidarianswhich of the following pairs of terms is mismatchedwhich objective lens has the shortest working distanceolfactory cells and taste buds are normally stimulated byundifferentiated spermatogenic cells are calledaction potential na k pumpkills cancer cells and virus infected body cellssavanna temperature rangeequilibrium receptors are located in theround periodic tableconical projectionsmicrocytic hypochromic anemia with eosinophiliahip hiking definitioncharacteristic of protistapsvt ekgactions of sternocleidomastoidwhat happens when a cell transcribes a viral geneneck rightingpivot joint motionplant fertilization diagrammost skeletal muscles contain ________blotting techniques in biotechnologyanatomy physiology saladindefine axial skeletonis ethanol water solubleindependent assortment of chromosomes is a result ofwhat organic compound contains a hydroxyl groupmrna codesphenergan nursing implicationscellular respiration easystructure and function of tonsilscultural anthropology a toolkit for a global age pdfvals motorstelophase 1 meiosisexhaled co2to kill a mocking bird quizsocl2 reagentkeystone of the craniumalkaline urine uticartilaginous jointwhat are the stop and start codonsfunction of pancreatic juice in digestionantonym for quiverpatellar knee jerk reflexarises from peripheral or incidental transactionsis the spleen a lymph nodestephen nathanson an eye for an eyekobe earthquake secondary effectssuffixes medical terminologywhen do chromosomes become visibleepidermal layers in ordertypes of flat boneswhat does insoluble fiber refer tonew world encyclopedia orgduring lactic acid fermentationviv latin rootosteoblasts and osteoclastsnormal depth and rate of respirationexternal sphincter of bladderwhat is the choroidcharacters of crucibleribonucleotides and deoxyribonucleotidesmetaproterenol side effectscodominant punnett squarespinal cord spinal nervesadlerian counseling techniquesspanish weather flashcardsperiodic table flash cardsmicrotubules in animal cellembalming terms