Gram + Unknowns MSA, TSA, 6.5% NaCl 45degree, Trehalose

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1

Gram + unknown for what genus?

Staphylococcus

2

How many different unknown species of Gram+ are there?

6

3

how many different tests to ID the unknown are there?

9

4

What does Mannitol Salt Agar detect for?

fermentation of mannitol

5

what does the fermentation of MSA produce?

acid

6

What is the pH indicator of MSA?

phenol red

7

what color will the MSA turn if it is acidic?

yellow

8

If the MSA does not change color and remains red what is the pH?

basic

9

What is trehalose?

a disaccharide of glucose ( alpha-1,1 linkage)

10

what does Trehalose do?

ferment to produce acid

11

What is the pH indicator for Trehalose agar?

phenol red

12

If the unknown for Trehalose agar is yellow what does it indicate ?

acid, ferments to produces acid

13

If the unknown for Trehalose agar is red what does it indicate ?

basic

14

What is the growth in hypertonic media solution ?

6.5% NaCl

15

what does the 6.5% NaCl broth testing?

if the organism survives it will ferment and produce acid

16

what is the pH indicator for 6.5% NaCl broth ?

bromocresol purple

17

If the NaCl broth turns purple what is the pH?

alkaline?

18

what color will the NaCl broth turn if it is neutral?

brown, weak fermenter

19

if the NaCl turns yellow what does it indicate?

positive fermenter and acidic

20

How is the temperature of 45 degree Celsius ?

hot, 113 degree Fahrenheit

21

What is so different with the NaCl and 45 degree C broths?

shorter incubation, 24 Hrs

22

in the 113 degree Fahrenheit is it easy to survive?

no

23

is organism survives 45 degree celsius what will it look like ?

cloudy (turbid), positive

24

if the organism dies in the 45 degree celsius what will it look like?

clear, negative

25

what tool did we use for the one fresh TSB ?

1 loop

26

what tool did we use for the MSA plate ?

loop

27

What was used for the Trehalose agar?

needle

28

what tool was used for the 6.5% NaCl broth?

swab

29

what tool was used for the 45 degree celsius TSB?

swab

30
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positive fermentation of mannitol, produce acid

31
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negative for fermentation of mannitol

32
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Turbid vs Clear


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