Lab 20 carbs fermentation and bgalactosidase activity
Resolving power, the ability to show detail, smaller wavelength greater detail
Magnifying power- ability to make objects appear larger
Parfocal- diff lenses that focus on same image and can be switched without adjusting the microscope as you move through lenses should only use fine
Differentiate between the resolving power and magnifying power of a lens. What is meant by the term parfocal?
Bc the shortest lens is the least likely to be smashed against a slide
Why is the low power objective placed in position when the microscope is stored or carried?
Oil has same optical qualities of glass and increases numerical aperture, keeps light from scattering, if no oil only have half a lense
Why is oil necessary when using 90x to 100x objective?
Iris diaphragm controls the amount of light passing through the slide or specimen and substage condenser focuses a cone of light on the slide or specimen
What is function of iris diaphragm? The substrate condenser?
It's the shortest distance between two parts on a specimen that can be distinguished by the observer
What is meant by the limit of resolution
Rheostat is a dimmer, start with lowest power on rheostat and it allows for slow ramping up of power instead of turning lamp on to highest and hottest setting
How can you increase the bulb life of your microscope if the bulbs voltage is regulated by a rheostat?
All the way up bc you are looking at very small things
What position should you keep your substage condenser at?
What are the 3 bacterial shapes observed?
Make sure lense clean including condenser
Scope must be properly adjusted
How can you increase resolution on microscope?
100x bc looking at organisms that at micrometers in size
In micro, what is most common used objective? Explain
10x if higher ocular would have more mag but wouldn't be able to see detail
In micro what is most common ocular used? Explain
5x * 10x. 50x
* 20x. 100x
If 5x instead of 10x ocular a used with same objectives, what mags would be achieved?
Ocular micrometer is a series of lines, to calibrate u need to line up at each lense
Why is it necessary to calibrate the ocular micrometer with each objective?
In the prepared slides, which organism was largest
The specimen is alive in a wet mount and if you heat fixed an organism it shrinks and is not an accurate measurement
When identifying microorganisms why should a wet mount be used to make messurements
Microscope slide with scale marked on surface, tiny ruler embedded in glass
What is a stage micrometer