Gram + Unknowns MSA, TSA, 6.5% NaCl 45degree, Trehalose
Gram + unknown for what genus?
How many different unknown species of Gram+ are there?
how many different tests to ID the unknown are there?
What does Mannitol Salt Agar detect for?
fermentation of mannitol
what does the fermentation of MSA produce?
What is the pH indicator of MSA?
what color will the MSA turn if it is acidic?
If the MSA does not change color and remains red what is the pH?
What is trehalose?
a disaccharide of glucose ( alpha-1,1 linkage)
what does Trehalose do?
ferment to produce acid
What is the pH indicator for Trehalose agar?
If the unknown for Trehalose agar is yellow what does it indicate ?
acid, ferments to produces acid
If the unknown for Trehalose agar is red what does it indicate ?
What is the growth in hypertonic media solution ?
what does the 6.5% NaCl broth testing?
if the organism survives it will ferment and produce acid
what is the pH indicator for 6.5% NaCl broth ?
If the NaCl broth turns purple what is the pH?
what color will the NaCl broth turn if it is neutral?
brown, weak fermenter
if the NaCl turns yellow what does it indicate?
positive fermenter and acidic
How is the temperature of 45 degree Celsius ?
hot, 113 degree Fahrenheit
What is so different with the NaCl and 45 degree C broths?
shorter incubation, 24 Hrs
in the 113 degree Fahrenheit is it easy to survive?
is organism survives 45 degree celsius what will it look like ?
cloudy (turbid), positive
if the organism dies in the 45 degree celsius what will it look like?
what tool did we use for the one fresh TSB ?
what tool did we use for the MSA plate ?
What was used for the Trehalose agar?
what tool was used for the 6.5% NaCl broth?
what tool was used for the 45 degree celsius TSB?
positive fermentation of mannitol, produce acid
negative for fermentation of mannitol
Turbid vs Clear